Unraveling the biochemical aspects of the interaction between ticks and <em>Leishmania</em> using a tick cell line

Leishmaniasis comprises a group of vector-borne neglected tropical diseases caused by species of the obligatory intracellular parasite Leishmania, transmitted by the bite of dipteran sand flies. Infected dogs serve as the primary domestic reservoir of Leishmania parasites and are often found in close association with various arthropods, such as fleas and ticks. There have been recent reports of Leishmania infections occurring in areas non-endemic for sand fly species, leading to reconsideration of the hypothesis that other arthropods, such as ticks, may also play a significant role in the natural history and epidemiology of leishmaniasis. Here, we used a tick cell line as a tool to study Leishmania infantum and tick interaction. The results showed that L. infantum can bind and proliferate inside Ixodes scapularis IDE8 tick cells. The infection reduced tick cell viability and induced ROS production. Lipid profile analysis showed that the presence of L. infantum increased oxysterol in tic..., Parasite Leishmania infantum (MCAN/BR/2008/1112), originally isolated in 2008 from a dog in Brazil, was maintained in Schneider’s insect medium supplemented with 10% fetal bovine serum (FBS) and 50 µg/mL gentamicin (Sigma) at 26 °C or Schneider’s insect medium supplemented with 10% FBS, 2% human urine, and 50 µg /mL gentamicin (Sigma) at 26 °C, in sealed T25 tissue culture flasks with weekly subculture. Tick Cell Line The I. scapularis tick cell line IDE8 was cultured in sealed T25 flasks in 5 mL of complete L15B medium as described previously&nbsp;(Marotta et al., 2018), at 32 °C. Leishmania** - Tick Cells Interaction In Vitro** IDE8 tick cells (2 x 105 per well) were seeded onto round glass coverslips in 24-well plates and maintained at 34 °C overnight. After this period, the cells were incubated with L. infantum promastigotes at a multiplicity of infection of 5 parasites to 1 cell (MOI 5) for 2 h.&nbsp;Following the incubation, free parasites were removed by washing with PBS, and the..., # Data from: Unraveling the biochemical aspects of the interaction between ticks and *Leishmania* using a tick cell line Dataset DOI: [10.5061/dryad.dv41ns2bb](10.5061/dryad.dv41ns2bb) ## Description of the data and file structure This dataset contains experimental data from Filgueiras et al (2025), testing the hypothesis that tick cell could interact with the *Leishmania* parasite and that the presence of the parasite cause biochemical alterations in tick cells. Each file corresponds to each figure of the paper, containing : (1) tick cell and *Leishmania* interaction in different time points, Figures 2 (A, B ,C), (2) Biochemical results, Figure 3 (A-C), (3) Lipid metabolism (Figures 4 A-G) ### Files and variables #### File: Figure_2_A.xlsx **Description:** Tick cells were incubated with *Leishmania* parasite. The percentage of infected- tick cell was determined at 2, 24 and 48 hours by counting at least 200 fixed and stained cells under an optical microscope ##### Variables * ...,利什曼病是一类经媒介传播的被忽视热带病，由专性细胞内寄生虫利什曼原虫（Leishmania）属物种引发，通过双翅目白蛉的叮咬进行传播。受感染的犬类是利什曼原虫的主要家畜保虫宿主，常与跳蚤、蜱虫等多种节肢动物密切共存。近期有研究报道称，在白蛉非流行区域也出现了利什曼原虫感染病例，这促使学界重新审视“蜱虫等其他节肢动物可能在利什曼病的自然史与流行病学中发挥重要作用”这一假说。本研究以蜱细胞系为工具，探究婴儿利什曼原虫（Leishmania infantum）与蜱虫的相互作用。结果显示，婴儿利什曼原虫可在肩突硬蜱（Ixodes scapularis）IDE8蜱细胞内黏附并增殖；感染会降低蜱细胞活力，并诱导活性氧（Reactive Oxygen Species, ROS）产生。脂质组分析表明，婴儿利什曼原虫的存在可提升蜱细胞内氧甾醇含量…… 婴儿利什曼原虫菌株（MCAN/BR/2008/1112）于2008年从巴西的一只病犬体内分离得到，分别采用两种培养体系：于26℃下，在添加10%胎牛血清（Fetal Bovine Serum, FBS）与50 μg/mL庆大霉素（Sigma公司）的Schneider昆虫培养基中培养；或于26℃下，在添加10%胎牛血清（FBS）、2%人尿液与50 μg/mL庆大霉素（Sigma公司）的Schneider昆虫培养基中培养，均置于密封的T25细胞培养瓶内，每周传代一次。 ### 蜱细胞系 肩突硬蜱IDE8细胞系于32℃下，在密封T25培养瓶中以5 mL完全L15B培养基培养，培养方法参照此前研究（Marotta等，2018）。 ### 体外利什曼原虫-蜱细胞相互作用实验 将IDE8蜱细胞（每孔2×10^5个）接种至24孔板内的圆形盖玻片上，于34℃下孵育过夜。随后以感染复数（Multiplicity of Infection, MOI）为5（即每个细胞对应5个寄生虫）的比例，与婴儿利什曼原虫前鞭毛体共孵育2小时。孵育结束后，用PBS洗涤去除游离的寄生虫…… # 数据来自：利用蜱细胞系解析蜱虫与利什曼原虫相互作用的生化机制 数据集DOI：[10.5061/dryad.dv41ns2bb](10.5061/dryad.dv41ns2bb) ## 数据与文件结构说明 本数据集包含Filgueiras等人（2025）的实验数据，用于验证“蜱细胞可与利什曼原虫相互作用，且寄生虫的存在会引发蜱细胞的生化改变”这一假说。 每个文件对应论文中的一幅图表，具体包括：(1) 不同时间点的蜱细胞与利什曼原虫相互作用情况，对应图2（A、B、C）；(2) 生化检测结果，对应图3（A-C）；(3) 脂质代谢相关检测结果，对应图4（A-G）。 ### 文件与变量说明 #### 文件：Figure_2_A.xlsx **描述：** 蜱细胞与利什曼原虫共孵育后，分别在2、24、48小时通过光学显微镜计数至少200个经固定与染色的细胞，以确定感染蜱细胞的百分比。 ##### 变量 * ……