Plant responses to and microbial community composition of greenhouse soils growing Phragmites australis

To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify the effects of each plant lineage on soil microbes and link plant responses to microbial communities. Specifically, DNA was extracted from soils and fungal and bacterial DNA was amplified to identify the microbial constituents. Amplicons were sequenced using Illumina MiSeq. This dataset includes outputs of bioinformatic analysis of sequences including operational taxonomic unit (OTU) generation, OTU abundance, resolved taxonomy, and environmental metadata collected in our survey. Raw sequences were uploaded to the NCBI Sequence Read Archive under SRA accession number PRJNA719385.

为明确普通芦苇（Phragmites australis）的本土与外来谱系如何影响土壤细菌、真菌及卵菌，并对其产生响应，我们从美国密歇根州与俄亥俄州的10个采样点，分别采集了对应本土与外来芦苇谱系的活体根状茎、种子及土壤样本。我们对野外采集的样本进行扩繁培育，以开展带有多重微生物抑制处理的互惠移植式植物-土壤反馈实验。具体而言，我们探究了各芦苇谱系在经不同芦苇谱系预驯化的土壤，以及预灭菌土壤中的生长状况。植物生物量是评估植物对土壤微生物预驯化响应的核心观测变量。此外，我们采用DNA元条形码测序（DNA meta-barcoding）技术，解析各植物谱系对土壤微生物的调控效应，并将植物响应与微生物群落进行关联分析。具体实验流程为：从土壤样本中提取总DNA，扩增真菌与细菌的DNA以鉴定土壤微生物组成；扩增子采用Illumina MiSeq测序平台完成测序。本数据集包含序列的生物信息学分析结果，具体包括操作分类单元（operational taxonomic unit, OTU）生成、OTU丰度、分类学注释结果，以及本研究调查采集的环境元数据。原始序列已上传至美国国家生物技术信息中心（NCBI）的序列读取档案（Sequence Read Archive, SRA），获取的登录号为PRJNA719385。