Expression levels of target genes by Real-Time RT-PCR.

HEY, OVCAR5 and UCI-101 cells were treated for 24 hours with 300 µM of diclofenac or indomethacin. RNA was isolated and RT-qPCR performed. Shown is the average of 3 independent experiments. Numbers reflect the fold change difference in the mRNA levels in the treated samples, as compared to the untreated samples, after normalization with GAPDH. Statistical significance is represented as * p<0.05; † p<0.001.

将HEY、OVCAR5及UCI-101细胞系以300 μM浓度的双氯芬酸（diclofenac）或吲哚美辛（indomethacin）孵育24小时。随后提取总RNA并进行逆转录实时定量聚合酶链反应（RT-qPCR）实验。本次展示的数据为3次独立重复实验的平均值。所有数值代表经甘油醛-3-磷酸脱氢酶（GAPDH）归一化后，处理组与未处理组样本的mRNA水平倍数变化差异。统计学显著性标记规则为：* 表示p<0.05；† 表示p<0.001。