MICROBIOLOGICAL ANALYSIS OF A MUNICIPAL DUMPSITE AND IT'S ENVIRON IN LAGOS STATE, NIGERIA

Indiscriminate waste discharge and improper management of waste disposal facilities remains
Nigeria's biggest environmental concerns as it poses substantial risk to both public health and
ecosystem. This study explored how waste dumpsite affects the environment and public health. Soil,
leachate, borehole water and air samples collected from a dumpsite situated in Kosefe Local
Government in Lagos State, Nigeria and its environs were microbiologically analyzed. Microbial
isolation and enumeration utilized Nutrient, Eosin Methylene Blue, Salmonella Shigella agar and
MacConkey agar. Bacterial characterization was done phenotypically using morphological and
biochemical techniques. Antibiotic susceptibility employed Kirby-Bauer disk diffusion Soil had the
7 7 highest bacteria count (3.0×10 CFU/g), followed by leachate (1.48x10 CFU/ml), and borehole
6 6 3 water (5.2x10 CFU/ml), while air had the least bacterial count (4.0x10 CFU/m ). Thirty two
bacterial species belonging to seven genera were isolated and identified. Bacillusspp. occurred in all
samples asides water, while Salmonella spp. and Escherichia coli were found in leachate and
borehole water. Antibiotic resistance profile revealed that all the isolates were susceptible to
gentamicin and ofloxacin while 38% were resistant to amoxicillin and 56% of the isolates were
resistant to ceftriaxone. This calls for an urgent regulatory system on proper waste disposal in that area. Individuals living in close proximity to dumpsites are at high risk of infections caused by bacterial pathogens.

随意排放废物及废物处理设施管理不当仍是尼日利亚最严峻的环境问题，因其对公众健康和生态系统均构成重大风险。本研究探讨了垃圾填埋场如何影响环境和公众健康。研究人员从尼日利亚拉各斯州科塞费地方政府辖区内的一处垃圾填埋场及其周边采集了土壤、渗滤液、井水和空气样本，并对这些样本进行了微生物学分析。微生物分离与计数采用了营养琼脂（Nutrient agar）、伊红美蓝琼脂（Eosin Methylene Blue agar）、沙门氏菌-志贺氏菌琼脂（Salmonella Shigella agar）和麦康凯琼脂（MacConkey agar）。细菌鉴定采用表型方法，结合形态学和生化技术进行。抗生素敏感性测试采用Kirby-Bauer纸片扩散法（Kirby-Bauer disk diffusion）。土壤中的细菌数量最高（3.0×10^7 菌落形成单位/克，CFU/g），其次是渗滤液（1.48×10^7 菌落形成单位/毫升，CFU/ml）和井水（5.2×10^6 菌落形成单位/毫升，CFU/ml），而空气中的细菌数量最低（4.0×10^3 菌落形成单位/立方米，CFU/m³）。共分离并鉴定出属于7个属的32种细菌。芽孢杆菌属（Bacillusspp.）存在于除水以外的所有样本中，而沙门氏菌属（Salmonella spp.）和大肠杆菌（Escherichia coli）则在渗滤液和井水中被检出。抗生素耐药性分析显示，所有分离菌株均对庆大霉素和氧氟沙星敏感，而38%的菌株对阿莫西林耐药，56%的菌株对头孢曲松耐药。这表明该地区亟需建立完善的废物处理监管体系。居住在垃圾填埋场附近的居民面临细菌病原体感染的高风险。