Expression data from regulatory T cells in mice. Mus musculus

It is well accepted that the generation and functional stability of Treg cells stand as one dominant force to maintain immunological homeostasis during steady-state or under inflammatory conditions, as the primary Treg cell deficiency causes numerous types of autoimmunopathy, hypersensitivity or inflammation-induced carcinogenesis. We used microarrays to indentify the global programme of gene expression underlying regulatory mechanism and explore the differentially expressed genes during the process of immunology regulation. Overall design: WT or KO naïve T cells were freshly isolated from spleen and stimulated under Treg-induction conditions for 3 days. Cells were collected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain differential genes between WT and KO Treg cells.

目前学界普遍认为，调节性T细胞（Treg cells）的生成与功能稳定性，是维持生理稳态或炎症状态下免疫稳态的核心驱动力之一；而Treg细胞的原发性缺失会引发多种自身免疫病、超敏反应或炎症诱导的癌变。本研究采用基因芯片（microarrays）技术，鉴定免疫调控机制背后的全局基因表达程序，并探究免疫调控过程中的差异表达基因。实验设计概述：从脾脏中新鲜分离野生型（WT）或敲除型（KO）初始T细胞，在Treg细胞诱导条件下刺激培养3天。收集细胞进行RNA提取，并在Affymetrix基因芯片上完成杂交反应。本研究旨在筛选WT与KO Treg细胞之间的差异表达基因。