Transcriptomic analysis of Pot1a/b knockout in a native mouse model of glioma

Please see the cited publication below for further details. This study found that Pot1a/b knockout in a native mouse model of glioma confers sexual dimorphism of survival and discovered sex-dependent changes in telomere content as well as signatures of immune infiltration and cell cycle in these tumors. Overall design: The native mouse model of glioma used in this study (C3 model) was generated via embryonic CRISPR/Cas9 knockout of Trp53, Nf1, and Pten. Additional CRISPR/Cas9 knockout of Pot1a and Pot1b resulted in the C5 model.

欲了解更多细节，请参阅下文引用的文献。本研究发现，在胶质瘤天然小鼠模型中敲除Pot1a/b基因可导致生存期呈现性别二态性，并揭示了此类肿瘤中端粒含量的性别依赖性变化，以及免疫浸润与细胞周期特征。实验整体设计：本研究使用的胶质瘤天然小鼠模型（C3模型）通过胚胎期CRISPR/Cas9基因敲除Trp53、Nf1及Pten基因构建得到；在此基础上额外对Pot1a与Pot1b基因进行CRISPR/Cas9基因敲除，即可构建得到C5模型。