Genes required for M. xanthus fruiting body development.

1Codon bias was determined using the software available at the Computational Microbiology Laboratory http://www.cmbl.uga.edu/software.html. The larger the number the greater the deviation from normal M. xanthus codon usage. Genes with no entries have a codon bias consistent with other M. xanthus genes.2tBLASTn algorithm was used to identify the four most closely related homologs. Each gene was classified as δ Proteobacteria if at least one of the four top homologs belongs to an organism in that group. Gene was classified as unique based on a 1e−10 cutoff. Gene was classified as ‘other’ if the closest four homologs belonged to another phylogenetic group.3Code refers to the classification category. Proteins known to be involved in signal production or sensory transduction are denoted with an ‘S’. The remaining genes were examined for a putative enzymatic function in the annotation, which is indicated by an ‘E’

1. 密码子偏好性（codon bias）通过计算微生物学实验室（Computational Microbiology Laboratory，http://www.cmbl.uga.edu/software.html）提供的软件进行计算。数值越大，代表与粘球菌（M. xanthus）标准密码子使用模式的偏差程度越高。无对应条目的基因，其密码子偏好性与其他粘球菌基因保持一致。2. 采用tBLASTn算法识别与目标基因亲缘关系最近的4个同源基因。若4个最优同源基因中至少有一个隶属于δ-变形菌门（δ Proteobacteria）类群，则将该基因归类为δ-变形菌门相关基因；以1e−10为阈值将基因判定为“独特基因”；若4个最近缘同源基因属于其他系统发育类群，则将该基因归类为“其他”类。3. 分类编码（Code）指代上述分类类别。已知参与信号生成或感知转导的蛋白质以符号“S”标注；剩余基因若在注释中被预测具有潜在酶促功能，则以符号“E”标注。