scRNA-seq over multiple time points of heart development in WT C57Bl6/J embryos and in Tbx1 mutant mice (Tbx1 KO)

We performed scRNA-seq over multiple time points of heart development in WT C57Bl6/J embryos and in Tbx1 mutant mice (Tbx1 KO). We dissected primarily the cardiac region but also the regions dorsal to the heart and the pharyngeal arches to capture the progenitor cells that migrate into the heart and the neural crest cells. For time points E10.5 and E11.5, we primarily dissected the regions behind the heart and included less of the overall cardiac region. We included pharyngeal arches for all time points and, at E11.5, we excluded the first arch. For Tbx1 null embryos, we also generated scRNA-seq data for WT and mutant embryos from the same pool of dissociated cells used for scATAC-seq. Overall design: For all embryos, we micro-dissected the tissue and dissociated using TryplE. For all samples, multiple replicates for WT and KO were used and we include all replicates below.

本研究针对野生型C57BL/6J（Wild Type，WT）胚胎以及Tbx1突变小鼠（Tbx1敲除，Tbx1 KO）的心脏发育多个时间节点，开展了单细胞RNA测序（scRNA-seq）。实验中我们主要取材心脏区域，同时也采集心脏背侧区域与鳃弓组织，以捕获迁移至心脏的祖细胞及神经嵴细胞。针对E10.5与E11.5两个发育时间点，我们主要解剖心脏后方区域，整体心脏区域的取材占比相对更低。所有时间节点的样本均包含鳃弓组织，但在E11.5时间点我们排除了第一鳃弓。针对Tbx1纯合缺失胚胎，我们还使用了与单细胞ATAC测序（scATAC-seq）相同的解离细胞池，完成了野生型与突变体胚胎的scRNA-seq数据生成。整体实验设计：所有胚胎的组织均经显微解剖后，使用TrypLE试剂进行细胞解离；所有样本均设置了多组野生型与敲除型生物学重复，下文将呈现全部重复样本的相关数据。