Data from 'Deoxygenation lowers thermal threshold of coral bleaching.'

Increasing exposure to deoxygenation as a result of climate warming and localised pollution is emerging as an important contributing factor for widespread coral bleaching and mortality. Some corals have been observed to thrive under multiple extreme conditions including very low O2 levels and high temperatures yet the combined effects of heating and deoxygenation on coral bleaching susceptibility remain unknown. Here, we incorporated deoxygenated seawater into short-term heat assays (CBASS) to show that deoxygenation can lower the thermal limit of an Acropora coral species. 

Data files provided:

'CBASS_deoxy_ref_transcriptome.fasta' contains the reference transcriptome assembled de novo using SOAPdenovo-Trans using non-bleached samples of the CBASS assay (i.e., 30°C and 33°C) and then filtered to keep only reads ≥ 500 bp in length (20,115 contigs).

'CBASS_deoxy_BIS_photos_Initial_and_poststress.pdf' contains images of coral fragments alongside a colour reference chart for estimating bleaching index scores (BIS) before experiment starts (initial) and post 6hr stress phase. Sample IDs are denoted as: H0/HH = normoxia/deoxygenation treatment, 30/33/36/39 = temperature in °C, T1 = time point 1, R1/2/3/4 = replicate 1/2/3/4.

'CBASS_deoxy_SymPortal_output_files.zip' contains all SymPortal (https://symportal.org) output files.

随着气候变暖与局地污染加剧，海洋脱氧现象愈发普遍，正成为引发大规模珊瑚白化与死亡的重要诱因之一。现有观测表明，部分珊瑚可在极低氧浓度与高温等多重极端环境下存活，但升温与脱氧的联合作用对珊瑚白化易感性的影响仍未明确。本研究将脱氧海水纳入短期热应激实验（CBASS），结果证实脱氧作用可降低轴孔珊瑚（Acropora）物种的热耐受上限。

提供的数据文件如下：

"CBASS_deoxy_ref_transcriptome.fasta"包含经SOAPdenovo-Trans软件从头组装的参考转录组，该转录组基于CBASS实验的非白化样本（即30°C与33°C组）构建，后续经筛选仅保留长度≥500 bp的序列，共得到20115条重叠群（contig）。

"CBASS_deoxy_BIS_photos_Initial_and_poststress.pdf"包含珊瑚片段与比色卡的实拍图像，用于评估实验开始前（初始状态）及6小时应激阶段后的白化指数评分（BIS）。样本编号规则如下：H0/HH代表常氧/脱氧处理组，30/33/36/39代表温度（单位：°C），T1代表时间点1，R1/2/3/4代表重复组1至4。

"CBASS_deoxy_SymPortal_output_files.zip"包含SymPortal（https://symportal.org）的全部输出文件。