Natural mutations in the sensor kinase of the PhoPR two-component regulatory system modulate virulence of ancestor-like tuberculosis bacilli

In Mycobacterium tuberculosis, the PhoPR two-component regulatory system controls production and secretion of proteins and lipid virulence effectors. Several mutations, present in phoR of Mycobacterium canettii relative to M. tuberculosis, impact the expression of the PhoP regulon and the pathogenicity of the strains. Here, we analyse by RNA-seq the expression profile of PhoP-regulated genes between the two M. tuberculosis strains H37Rv and HN878 and the two M. canettii isolates STB-Ks and STB-Kr. Overall design: Gene expression profiling by RNA-seq of M. tuberculosis H37Rv and HN878 strains and M. canettii STB-Ks and STB-Kr isolates cultured in normal growth condition

结核分枝杆菌（Mycobacterium tuberculosis）中，PhoPR双组分调控系统（two-component regulatory system）可调控蛋白质与脂质毒力效应因子的产生与分泌。相较于结核分枝杆菌，斯氏分枝杆菌（Mycobacterium canettii）的phoR基因携带多种突变，这些突变可影响PhoP调节子（PhoP regulon）的表达以及菌株的致病性。本研究通过RNA测序（RNA-seq）分析了两株结核分枝杆菌菌株H37Rv、HN878，以及两株斯氏分枝杆菌分离株STB-Ks与STB-Kr的PhoP调控基因表达谱。实验整体设计：将结核分枝杆菌H37Rv、HN878菌株与斯氏分枝杆菌STB-Ks、STB-Kr分离株置于正常培养条件下培养，通过RNA-seq开展基因表达谱分析。