LDDN-0003499 treatment attenuated active, phosphorylated Lyn and Src but not ERK, JNK, or p38 protein levels in BV2 cells.

Microglial BV2 cells were treated with vehicle (DMSO), 0.5nM, 5nM, 50nM, 0.5 μM, 5μM, and 50 μM LDDN-0003499 for 1h. Cells lysates were used for western-blot analyses with (B) anti-pLyn (Tyr 396), (C) anti-pSrc (Tyr 416), (D) anti-pERK, (E) anti-pJNK, and (F) anti-p-p38 antibodies with Lyn, Src, ERK2, JNK, and p38 antibodies as their respective loading controls. Optical densities from three independent experiments were graphed and averaged ± SD (*p<0.05 vs. vehicle). (A) A representative western blot is shown.

将小胶质细胞BV2细胞（Microglial BV2 cells）分别以溶剂对照（二甲基亚砜，DMSO）、0.5nM、5nM、50nM、0.5μM、5μM及50μM的LDDN-0003499处理1小时。收集细胞裂解液用于蛋白质免疫印迹分析：(B) 使用抗磷酸化Lyn（酪氨酸396）抗体、(C) 抗磷酸化Src（酪氨酸416）抗体、(D) 抗磷酸化ERK抗体、(E) 抗磷酸化JNK抗体以及(F) 抗磷酸化p38抗体进行检测，以Lyn、Src、ERK2、JNK及p38抗体作为各自的内参对照。对三次独立重复实验得到的光密度值进行绘图并计算平均值±标准差（*p<0.05，与溶剂对照组相比）。(A) 展示了一张代表性的蛋白质免疫印迹结果。