Summary of genotypes examined.

Listing of animal genotypes examined and the temperature regimen under which their germline cells were investigated. The majority of the genotypes were shifted to a different temperature as one to seven day old adults. Testes were dissected seven days after the temperature shift. stet or spi mutant animals without expression constructs were raised to adulthood and dissected within one to seven days after hatching. For each genotype, testes from at least 30 animals were examined. Though the severity of the phenotypes of the different genotypes varied, they all contained clusters of over-proliferating early-stage germline cells among otherwise wildtype appearing germline cells.

本数据集收录了本次研究中检测的动物基因型，以及对应其生殖细胞观测所采用的温度调控方案。绝大多数基因型的受试动物在成虫1至7日龄时被转移至不同温度环境培养，并于温度转换后的第7天完成睾丸解剖取材。未携带表达载体的stet或spi突变体动物则被饲养至成虫阶段，并于孵化后1至7日内完成解剖取材。针对每种基因型，至少取30只动物的睾丸进行观测分析。尽管不同基因型对应的表型严重程度存在差异，但所有样本的生殖细胞群体中均存在过度增殖的早期生殖细胞簇，其余生殖细胞则呈现野生型（wildtype）表型。