Listeria infection of Caco-2 cells. Homo sapiens

This experiment set contains all of the arrays described in Baldwin et al., Genome Biology, 2002. All experiments are time courses, with the exception of multiple measurements for the zero time point in the parallel time course for the purpose of zero transformation. The parallel four hour time course was performed on a single day and three independent measurements were taken prior to infection. The eight hour time courses were each performed on different days, with single time zero measurements. Each array represents a measurement of human mRNA from cultured intestinal epithelial cells (red channel), relative to the control reference pool of mRNA (green channel), at different time points during the course of an infection by Listeria monocytogenes, or the mutants actA (defective in actin-based motility) and prfA (defective in virulence factor gene expression). Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set Overall design: Computed

本实验集涵盖了Baldwin等人于2002年发表在《基因组生物学（Genome Biology）》中的全部基因芯片阵列数据。所有实验均为时间序列实验，仅平行时间序列实验的零时间点设置了多次重复测量，以用于零点校准转换。本次平行4小时时间序列实验于单日内完成，且在感染前采集了三次独立样本进行检测。所有8小时时间序列实验均于不同日期开展，且仅设置单次零时间点测量。每张芯片阵列均对应感染单核细胞增生李斯特菌（Listeria monocytogenes）、肌动蛋白基运动缺陷突变株actA，或毒力因子基因表达缺陷突变株prfA过程中，不同时间点的体外培养人肠上皮细胞的mRNA检测结果，以mRNA对照参考池为绿色通道，样本mRNA为红色通道，开展相对定量分析。本芯片阵列集依据共享的生物学背景（如物种、肿瘤类型、生物学过程等）进行归类整理。关键词：逻辑集合（Logical Set）。整体实验设计：经计算生成。