Transcriptional signature in liver reveal seasonal metabolic adaptation in the night-migratory blackheaded buntings. Transcriptional signature in liver reveal seasonal metabolic adaptation in the night-migratory blackheaded buntings

Purpose: Next-generation sequencing (NGS) has provided an opportunity to understand the underline processes invloved in migratory behavior. The goals of this study are to compare NGS-derived liver transcriptome profiling (RNA-seq) under migratory and non-migratory processes and understand the processes contributign under the physiological state. Overall design: In total, 12 liver samples (6 x 2; one sample from each of the six time-points of both M and nM states) were sequenced and mapped against zebra finch genome. A range of 20-30 million 100 base single-end reads were generated for each liver sample

研究目的：下一代测序（Next-generation sequencing, NGS）为解析鸟类迁徙行为背后的潜在调控过程提供了全新契机。本研究旨在对比迁徙与非迁徙状态下，基于NGS的肝脏转录组谱（RNA测序，RNA-seq）数据差异，进而阐明该生理状态下的核心分子调控机制。
整体实验设计：本研究共纳入12份肝脏样本（6×2组，即迁徙组（M）与非迁徙组（nM）各设置6个时间点，每组每个时间点各获取1份样本），将样本进行测序后比对至斑胸草雀（Zebra finch）参考基因组。每份肝脏样本均生成2000万至3000万条100碱基单端测序读段。