Expression profile of TTHA1939-deficient Thermus thermophilus HB8 strain

We observed the expression profile of the total mRNA of the TTHA1939-deficient strain grown in a rich medium at 70°C for 800 min. Wild-type and the mutant strains were precultured in rich medium (TR medium) for two times at 70 oC and then subcultured to minimum essential medium (CS medium). These cells were harvested during the logarithmic growth phase (800 minutes from the start of culture). Total RNA were extracted from each strain and used for the cDNA synthesis. For the biological replication, above experiments were performed four times independently.

本研究针对在丰富培养基中于70℃条件下培养800分钟的TTHA1939缺陷型菌株，检测其总mRNA（total mRNA）的表达谱。将野生型菌株与该突变株先在丰富培养基（TR medium）中于70℃下预培养两次，随后转接至最低必需培养基（CS medium）中继续培养。于对数生长期（培养起始后800分钟）收集上述细胞，从各菌株中提取总RNA并用于互补脱氧核糖核酸（complementary DNA, cDNA）的合成。为开展生物学重复实验，上述实验独立重复四次。