Targeting Taxane-Platin Resistant Lung Cancers with JumonjiC Lysine Demethylase Inhibitors (ChIP-Seq)

Characterization of histone 3 lysine 4 and lysine 27 trimethylation changes upon development of taxane-platin drug resistance in NSCLC cells and evaluation of these histone modifications after treatment with Jumonji KDM inhibitors, JIB-04 and GSK-J4. Comparison of H3K4me3 and H3K27me3 profiles between H1299 Parental cells (chemo-sensitive) and H1299 T18 cells (taxane-platin resistant), and assessment of Jumonji KDM inhibitors on H1299 T18 cells (JIB-04 treated vs DMSO treated, and GSK-J4 treated vs. DMSO treated).

本数据集旨在表征非小细胞肺癌（Non-Small Cell Lung Cancer, NSCLC）细胞在产生紫杉烷-铂类耐药性的过程中，组蛋白3赖氨酸4与赖氨酸27三甲基化修饰的变化情况，并评估经Jumonji家族组蛋白赖氨酸去甲基化酶（Jumonji KDM）抑制剂JIB-04与GSK-J4处理后，上述组蛋白修饰的改变。此外，本数据集还包含两项对比与评估实验：一是比较化疗敏感型亲本H1299细胞与紫杉烷-铂类耐药型H1299 T18细胞的H3K4me3与H3K27me3修饰谱；二是针对H1299 T18细胞开展Jumonji KDM抑制剂作用评估，分别设置JIB-04处理组与二甲基亚砜（Dimethyl sulfoxide, DMSO）对照组、GSK-J4处理组与二甲基亚砜（DMSO）对照组。