Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides

A difference in the transcriptomes of 8F10 T cells sourced from the NOD (8F10-NOD) or B16A (8F10-B16A) hosts was analyzed. As a result we found a number of biological pathways upregulated in the 8F10-NOD T cells including oxidative phosphorylation (OXPHOS), Myc targets, fatty acid metabolism, mTOR complex 1 (mTORC1) signaling. Overall design: T and B cell-depleted bone marrow stem cells from TCR alpha chain-deficient insulin peptide-specific 8F10 mice (CD45.2) were adoptively transferred into non-lethally irradiated NOD or B16A hosts (CD45.1). After 6 weeks of parking, the 8F10 T cells were FACS-sorted from inguinal lymph nodes of either host and subjected to RNA sequencing.