Search for genes that are expressed in female-specific, sex steroid-responsive peptidergic (FeSP) neurons of medaka (Oryzias latipes) in an ovarian-dependent manner

We isolated and purified female-specific, sex steroid-responsive peptidergic (FeSP) neurons from intact, sham-operated, and ovariectomized female medaka (Oryzias latipes) and performed RNA sequencing on these neurons (50 neurons per sample, n = 3 for each experimental group). Pairwise differential expression analysis between experimental groups identified genes that are expressed in FeSP neurons in an ovarian-dependent manner.

本研究从完整未处理、假手术及卵巢切除的雌性青鳉（Oryzias latipes）中分离纯化得到雌性特异性、性类固醇反应性肽能（FeSP）神经元，并对这些神经元开展RNA测序：每个样本包含50个神经元，每个实验组设置3个生物学重复。通过对各实验组进行两两差异表达分析，鉴定出在FeSP神经元中表达呈卵巢依赖性的基因。