Isoform sequencing of barley (Hordeum vulgare), using the reference cultivar 'Morex'.. ISOseq of barley (Hordeum vulgare, cultivar 'Morex')

Transcriptome sequencing with short read technology usually involves either de novo assembly or mapping of the reads to a reference sequence. Both of these approaches can result in bioinformatics artefacts that lead to the output of fragmented transcripts. To overcome this problem we chose a Pacific BioSciences IsoSeq approach which can theoretically yield full-length transcripts, without the need for assembly or read mapping. We applied this approach in barley (Hordeum vulgare), using the reference cultivar 'Morex'.

采用短读长测序技术的转录组测序，通常要么开展从头组装（de novo assembly），要么将测序读段（reads）比对至参考序列。这两种方法均可能产生生物信息学伪影（bioinformatics artefacts），进而导致片段化转录本的输出。为解决该问题，本研究选用太平洋生物科学公司（Pacific BioSciences）的IsoSeq技术，该技术理论上可获得全长转录本，无需进行组装或读段比对。本研究以大麦（Hordeum vulgare）的参考栽培品种‘Morex’为实验材料，应用了该技术。