The global wheat gene expressional responses to fusaoctaxin B. The global wheat gene expressional responses to fusaoctaxin B

Purpose: To assess global changes of wheat coleoptiles gene expression in response to fusaoctaxin B which is a linear, C-terminally-reduced and D-amino acid residue-rich octapeptide, as the product of the two non-ribosomal peptide synthetases encoded by Fusarium graminearum fg3_54 cluster. Methods: For RNA seq of wheat coleoptiles, three biological replicates of after 3 hours fusaoctaxin B-treated coleoptiles, after 3 hours control (0.5% Tween-20) treated coleoptiles, after 24 hours fusaoctaxin B-treated coleoptiles and after 24 hours control (0.5% Tween-20) treated coleoptiles were sequenced. Results: For each sample, 33-95 M RNA-seq clean reads were obtained mapped to the Triticum aestivum Chinesespring genome sequence. Conclusions: Fusaoctaxin B represents an unprecedented virulence factor. Overall design: Three biological replicates of after 3 hours fusaoctaxin B-treated coleoptiles, after 3 hours control (0.5% Tween-20) treated coleoptiles, after 24 hours fusaoctaxin B-treated coleoptiles and after 24 hours control (0.5% Tween-20) treated coleoptiles were sequenced using Illumina NovaSeq 6000 Sequencing System.

研究目的：本数据集旨在评估小麦胚芽鞘基因表达的全局变化，以响应镰孢毒素B（fusaoctaxin B）——该物质是一种线性、C端缩短且富含D-氨基酸残基的八肽，为禾谷镰孢菌（Fusarium graminearum）fg3_54基因簇编码的两种非核糖体肽合成酶（non-ribosomal peptide synthetases）的产物。
实验方法：针对小麦胚芽鞘的RNA测序实验中，对以下四组样本各设置3次生物学重复：3小时镰孢毒素B处理组胚芽鞘、3小时对照（0.5%吐温-20）处理组胚芽鞘、24小时镰孢毒素B处理组胚芽鞘、24小时对照（0.5%吐温-20）处理组胚芽鞘，均进行测序。
实验结果：每个样本可获得33~95百万条RNA测序清洁读段（clean reads），并将其比对至普通小麦（Triticum aestivum）中国春基因组序列。
研究结论：镰孢毒素B是一种前所未有的毒力因子。
整体实验设计：本研究对3小时镰孢毒素B处理组胚芽鞘、3小时对照（0.5%吐温-20）处理组胚芽鞘、24小时镰孢毒素B处理组胚芽鞘、24小时对照（0.5%吐温-20）处理组胚芽鞘各3次生物学重复样本，采用Illumina NovaSeq 6000测序系统完成测序。