Observational study to cross-validate the use of NG2 and PDGFRbeta as markers for vascular pericytes on murine cerebral capillaries.

Observational study to visualize and confirm NG2 and Platelet-Derived Growth Factor Receptor-beta (PDGFRb) localization on pericytes labeled by DsRed within murine cerebrovasculature from an Ng2/Cspg4:DsRed/+ animal (immediate collection, 0-hrs in culture). Images acquired on a Zeiss LSM 880 point-scanning confocal microscope to detect NG2 and PDGFRb co-localization on the pericytes within cerebral capillaries. Volumetric images taken through the z-axis were acquired at 40x. Images in the figure are compressed z-stacks within a selected range, but the raw image files in .czi format are also included.旨在可视化并验证NG2与血小板衍生生长因子受体β（Platelet-Derived Growth Factor Receptor-beta, PDGFRb）在Ng2/Cspg4:DsRed/+转基因小鼠脑血管系统中DsRed标记的周细胞（pericytes）上的定位的观察性研究（样本即时采集，未进行体外培养）。使用蔡司LSM 880点扫描共聚焦显微镜采集图像，以检测脑毛细血管内周细胞上NG2与PDGFRb的共定位情况。沿z轴采集的体积图像放大倍数为40倍。图中图像为选定范围内的压缩z-stack图像，但同时包含.czi格式的原始图像文件。