Gestational high salt stressed Embryonic Day 14.5 BdkrB2 Null Mouse Kidney vs. BdkrB2 Wild Type Mouse Kidney

Transcriptional profiling of Embryonic Day 14.5 mouse kidneys comparing the infuence of gestational high salt stress on gene expression remolding of BdkrB2 receptor null mice with that of BdkrB2 receptor wild type mice. The BdkrB2 receptor has been shown to be playing a role in renal vascular tone, kidney secretion and reabsorption function, normal kidney development, while impaired BdkrB2 receptor in kidney shown being associated with renal agenesis and renal dysplasia. Goal was to determine the effects of BdkrB2 receptor knockout together with gestational high salt stress on renal gene expression pattern. Two-condition experiment, BdkrB2 null mouse kidney vs. BdkrB2 WT mosue kidney with both on gestational high salt stress . Biological replicates: 3 BdkrB2 null/WT replicates, 3 BdkrB2 WT/null replicates, all 6 replicates were duplicated.

本数据集针对胚胎第14.5天的小鼠肾脏开展转录组分析（transcriptional profiling），旨在比较妊娠期高盐应激对缓激肽B2受体（BdkrB2 receptor）敲除小鼠与野生型小鼠肾脏基因表达重塑的影响。
已有研究证实，缓激肽B2受体参与调控肾脏血管张力、肾脏分泌与重吸收功能以及正常肾脏发育进程；而肾脏中该受体功能受损，已被证实与肾发育不全及肾发育异常相关。
本研究的核心目标为明确缓激肽B2受体敲除联合妊娠期高盐应激对肾脏基因表达谱的影响。
本实验设置两组对照：均暴露于妊娠期高盐应激环境下的缓激肽B2受体敲除小鼠肾脏与野生型（Wild Type，WT）小鼠肾脏。
生物学重复设置为：缓激肽B2受体敲除组与野生型组各3例生物学重复，全部6例样本均完成了重复实验。