Metabarcoding to Detect Invasive Fishes data

Data associated with a DNA metabarcoding study of fish (invasive and native) biodiversity in four rivers in Ontario Sub directories 00_sample_map: Map of sampling locations and abundance of larval fishes collected at each site 01_database_build: Reference genetic database and taxonomic information for classification of metabarcoding sequencing reads 02_qiime2_analysis: De-noising and dereplicating DNA metabarcoding data 03_read_correction: Correction for contamination using no-template controls and single fish samples 04_sampling_stats: General summary statistics of sequencing 05_river_richness: Summaries and analyses of species richness across rivers 06_gear_richness: Comparison of gear types on species detected 07_primer_consistency: Comparison of primer target gene on species detected 08_sampling_depth: Comparison of number of samples collected to read depth per sample 09_database_comparison: Comparison of the reference databases for COI and 12S 10_amplicon_distance: Comparison of the genetic distance for COI and 12S metabarcoding primers

本数据集关联一项针对安大略省四条河流中鱼类（入侵物种与本土物种）生物多样性的DNA宏条形码（DNA metabarcoding）研究，其子目录如下：
00_sample_map: 采样点位分布图及各站点采集的仔稚鱼丰度数据
01_database_build: 用于宏条形码测序读段分类的参考遗传数据库与分类学信息
02_qiime2_analysis: DNA宏条形码数据的去噪与去重复分析
03_read_correction: 基于无模板对照及单鱼样本的污染校正流程
04_sampling_stats: 测序实验的通用汇总统计信息
05_river_richness: 跨河流物种丰富度的汇总与分析
06_gear_richness: 采样渔具类型对物种检出效果的对比研究
07_primer_consistency: 引物靶基因差异对物种检出一致性的对比分析
08_sampling_depth: 采集样本量与单样本测序读深的对比分析
09_database_comparison: COI（细胞色素c氧化酶亚基I）与12S（12S核糖体RNA基因）参考数据库的对比分析
10_amplicon_distance: COI与12S宏条形码引物对应的扩增子遗传距离对比分析