c3bta_metadata

RNA sequencing (RNA-seq) technology has become increasingly important in precision medicine and clinical diagnostics, and emerged as a powerful tool for identifying protein-coding genes, performing differential gene analysis, and inferring immunecell composition. Human peripheral blood samples are widely used for RNA-seq, providing valuable insights into individual biomolecular information. Blood samples can be classified as whole blood (WB), plasma, serum, and remaining sediment samples, including plasma-free blood (PFB)and serum-free blood (SFB)samples that are generally considered less useful byproducts during the processes of plasma and serum separation, respectively. However, the feasibility of using PFB and SFB samples for transcriptome analysis remains unclear. In this study, we aimed to assess the suitability of employing PFB or SFB samples as an alternative RNA source in transcriptomic analysis. We performed a comparative analysis of WB, PFB, and SFB samples for different applications. Our results revealed that PFB samples exhibit greater similarity to WB samples than SFB samples in terms of protein-coding gene expression patterns, detection of differentially expressed genes, and immunological characterizations, suggesting that PFB can serve as a WB for transcriptomic analysis. Our study contributes to the optimization of blood sample utilization and the advancement of precision medicine research.

RNA测序（RNA-seq）技术已在精准医学与临床诊断中愈发重要，并成为识别蛋白质编码基因、开展差异基因分析以及推断免疫细胞组成的有力工具。人类外周血样本被广泛应用于RNA-seq研究，可为个体生物分子信息提供重要见解。血液样本可分为全血（whole blood, WB）、血浆、血清以及剩余沉淀样本，其中无血浆血液（plasma-free blood, PFB）与无血清血液（serum-free blood, SFB）样本通常分别被视为血浆分离与血清分离过程中产生的价值较低的副产物。然而，使用PFB与SFB样本进行转录组分析的可行性仍不明确。本研究旨在评估将PFB或SFB样本作为转录组分析中替代RNA来源的适用性。我们针对不同应用场景对WB、PFB与SFB样本开展了比较分析。本研究结果显示，在蛋白质编码基因表达模式、差异表达基因检测以及免疫学特征分析方面，PFB样本相较于SFB样本与WB样本具有更高的相似性，这表明PFB可作为转录组分析中WB的替代样本。本研究有助于优化血液样本的利用效率，并推动精准医学研究的发展。