Effect of benzimidazole derivative C162 on Staphylococcus aureus virulence. Staphylococcus aureus

From a Caenorhabditis elegans - S. aureus anti-infective screen, we identified benzimidazole derivative C162 as one of the potential anti-infective candidate that rescued the infected-nematodes from infection. This compound was found to exhibit anti-biofilm activity against S. aureus. To investigate the transcriptome profile of S. aureus in response to benzimidazole derivative C162, a genome-wide transcriptome analysis was performed on C162-treated S. aureus using the Affymetrix GeneChip S. aureus Genome Arrays. Our main interest is to look at the expression profiles of the biofilm-associated and virulence genes. Overall design: We conducted three independent microarray experiments (biological replicates) in the absence (control) and the presence (treated) of 6.25 µM benzimidazole derivative C162. We calculated the fold change as the ratio between the signal averages of three untreated and three C162-treated cultures at a single time-point (overnight incubation for 16-18 hours).

本研究通过秀丽隐杆线虫（Caenorhabditis elegans）-金黄色葡萄球菌（S. aureus）抗感染筛选模型，鉴定出苯并咪唑类衍生物（benzimidazole derivative）C162为潜在抗感染候选化合物之一，该化合物可使受感染线虫摆脱感染。
经检测，该化合物对金黄色葡萄球菌具有抗生物膜活性。
为探究苯并咪唑类衍生物C162处理后金黄色葡萄球菌的转录组表达谱，本研究采用Affymetrix GeneChip金黄色葡萄球菌基因组芯片，对经C162处理的金黄色葡萄球菌开展全基因组转录组分析。
本研究的核心关注点为生物膜相关基因与毒力基因的表达特征。
整体实验设计：我们设置了对照组（未添加C162）与处理组（添加6.25 μM苯并咪唑类衍生物C162），每组均开展3次独立的微阵列实验（生物学重复）。
在单一时间点（16~18小时过夜培养）下，以3份未处理培养物的信号平均值与3份C162处理培养物的信号平均值的比值计算折叠变化。