Best gene deletion strategies identified by OptORF using our MIP techniques for acetate production under glucose anaerobic condition.
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a‘Changes’ refer to the number of genes which are newly introduced in the solution with k deletions or removed from the solution with k–1 deletions.bYield is reported as % of the TMP for wildtype strain with a maximum glucose uptake rate of 10 mmol gDW−1 h−1 (2.56 mol acetate produced/mol glucose consumed). gDW stands for gram dry weight.
(a) 变化量(Changes)指在经k次基因敲除的方案中新增引入,或是在经k-1次基因敲除的方案中被移除的基因数量。
(b) 产物得率以葡萄糖最大摄取速率为10毫摩尔·克干重⁻¹·小时⁻¹的野生型菌株的TMP占比(%)进行报告,其中每消耗1摩尔葡萄糖可生成2.56摩尔乙酸。gDW为克干重(gram dry weight)的缩写。
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2015-12-02



