qPCR determination of TSPO mRNA levels in cells and tissue.

Cell and tissue mRNA were extracted, and TSPO and ARBP (house-keeping gene) mRNA levels determined by qPCR, as outlined in the material and methods section. Relative levels are expressed by normalized values using a Δct-based algorithm (ctTSPO-ctARBP). Fold increase compared to the lowest level of our sampling (brain) was calculated as outlined in the method section.

提取细胞与组织中的信使核糖核酸（mRNA），按照材料与方法部分所述实验流程，采用实时荧光定量聚合酶链式反应（qPCR）测定TSPO与ARBP（持家基因，house-keeping gene）的mRNA水平。相对表达水平采用基于循环阈值差（Δct = ct_TSPO - ct_ARBP）的归一化算法进行表征。以本次采样中表达水平最低的脑组织为参照，相对上调倍数的计算方法详见方法部分。