Genexpression of murine mesothelioma cell lines AC29 and AB1

RNA from two murine mesothelioma cell lines (AC29 and AB1) was extracted and hybridized to Affymetrix Microarrays to compare gene expression. Both mesothelioma cell lines were established following intraperitoneal introduction of crocidolite (asbestos) fibers (Davis et al. 1992) in CBA mice (AC29 cell line), and BALB/c mice (AB1). AB1 (passage #40) and AC29 (passage #60) cell lines were cultured in 3 T75 flasks per cell line and were harvested at 80% confluency after RNA was extracted from each flask and hybridized to Affymetrix Microarrays.

本数据集对应的实验处理如下：提取两种小鼠间皮瘤细胞系（AC29与AB1）的核糖核酸（RNA），并将其与Affymetrix基因芯片（Affymetrix Microarrays）进行杂交实验，以比较二者的基因表达差异。两种间皮瘤细胞系均通过向小鼠腹腔注射青石棉（crocidolite，即石棉）纤维诱导建立：其中AC29细胞系来源于CBA小鼠，AB1细胞系来源于BALB/c小鼠，相关构建细节参见Davis等人1992年的研究。本实验中，AB1细胞系（传代第40代）与AC29细胞系（传代第60代）均以每个细胞系接种3个T75培养瓶进行培养，待细胞汇合度达到80%时收获细胞；随后从每份培养瓶的细胞中提取RNA，并将其与Affymetrix基因芯片进行杂交实验。