MHC assembly (ace) file

De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for MHC classII beta (MHC). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, heterozygosity option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484

针对靶向主要组织相容性复合体（Major Histocompatibility Complex, MHC）II类β链的2个混合细菌人工染色体（Bacterial Artificial Chromosome, BAC）克隆，采用搭载长读长GS FLX Titanium化学试剂的454基因组测序仪FLX系统完成其焦磷酸测序数据的从头组装。本次分析使用罗氏（瑞士巴塞尔）开发的GS De novo Assembler软件，参数设置如下：启用大/复杂基因组选项与杂合性选项，以pCC1BAC HindIII载体序列作为修剪数据库，以大肠杆菌（Escherichia coli）菌株K12亚型DH10B基因组（GenBank登录号CP000948）作为筛选数据库，最小重叠长度设为200 bp，最小重叠相似度设为98%，最小重叠群（contig）长度设为500 bp。原始数据可在美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的序列读取档案（Sequence Read Archive, SRA）中获取，登录编号为SRP003484。