Effect of hypoxic stress on Estrogen receptor alpha binding to chromatin. Effect of hypoxic stress on Estrogen receptor alpha binding to chromatin

This project aimed at assessing the influence of hypoxic stress on Estrogen receptor alpha genome wide binding to chromatin, in presence or absence of E2 ligand. I also aimed at assessing if a 1-month hypoxic stress followed by 1 month of normoxia imprints an epigenetic memory, therefore altering genome wide ER binding (rescue condition). Overall design: 6 experimental conditions, with 1 technical replicate each 1) Normoxia : EtOH vs E2 (50 min); 2)Hypoxia: EtOH vs E2 (50 min); Rescue: EtOH vs E2 (50 min).

本项目旨在完成两方面研究目标：其一为评估缺氧应激（hypoxic stress）在存在或缺失雌二醇（Estradiol, E2）配体的条件下，对雌激素受体α（Estrogen receptor alpha, ERα）全基因组范围内与染色质结合的影响；其二为探究经1个月缺氧应激后复予1个月常氧处理的实验模型是否会形成表观遗传记忆（epigenetic memory），进而改变ERα的全基因组结合模式（该处理组为rescue条件，即恢复组）。本实验的整体设计如下：共设置6组实验条件，每组均包含1次技术重复（technical replicate），具体分组为：1) 常氧组：乙醇（Ethanol, EtOH）对照与E2处理组（处理时长50 min）的对比；2) 缺氧组：乙醇对照与E2处理组（处理时长50 min）的对比；3) 恢复组：乙醇对照与E2处理组（处理时长50 min）的对比。