N-terminome analysis of tomato P69D knock-out mutant total leaf proteomes treated with and without recominant P69D

The intercellular space or apoplast constitutes the main interface in plant-pathogen interactions. Apoplastic subtilisin-like proteases -subtilases- may play an important role in defence and they have been identified as targets of pathogen-secreted effector proteins. However, most evidence is limited to the interaction of plants with non-vascular foliar pathogens. Here, we characterise the role of the Solanaceae-specific P69 subtilase family in the interaction between tomato and the vascular bacterial wilt pathogen Ralstonia solanacearum.

细胞间隙或质外体（apoplast）是植物-病原体互作的主要界面。质外体类枯草杆菌蛋白酶（subtilases，subtilisin-like proteases）可能在植物防御过程中发挥重要功能，且已被鉴定为病原体分泌效应蛋白的靶标分子。然而，目前绝大多数相关证据仅局限于植物与非维管束叶部病原体的互作研究。本研究解析了茄科特异性P69枯草杆菌蛋白酶家族在番茄与维管束性青枯病病原菌青枯雷尔氏菌（Ralstonia solanacearum）互作过程中的功能。