Forest soil (Ah horizon) Metagenome. soil metagenome

In order to study the effects of drought exposed under heat stress on the bacterial community, 10 intact soil monoliths (50x50x70cm) with their native vegetation were sampled from a beech plot in the Hainich exploratory. After acclimatization to the greenhouse conditions, the monoliths were separated into soil moisture treatments (well-watered and drought, 5 replicates). To assess the community structure of the metabolically active bacteria, 16S rRNA was analyzed via MiSeq-based (Illumina) high throughput sequencing at three different time points (before and after drought treatment).

为探究热胁迫条件下干旱对细菌群落的影响，研究人员从海尼希试验样地的山毛榉林样地中采集了10块带有原生植被的完整土壤柱芯（soil monoliths，尺寸为50×50×70厘米）。经温室环境驯化后，将土壤柱芯分为两种土壤水分处理组：正常供水组与干旱组，每组设置5次生物学重复。为解析代谢活跃细菌的群落结构，研究人员在三个不同时间点（干旱处理前与干旱处理后），采用基于MiSeq（Illumina）平台的高通量测序技术对16S核糖体RNA（16S rRNA）进行测序分析。