RARg bookmarking functions in prostate cancer (IMPACT-Seq)

To define the RARg complex in PCa cell models, including isogenic variants of 22Rv1, how this is targeted by miR-96, and regulates the AR cistrome, and how this is specifically enriched in G2/M cells and how these actions collectively shape the transcriptional programs associated with PCa outcomes. Overall design: IMPACT-Seq was undertaken on HPr1AR or LNCaP cells transfected with 3' biotinylated miRIDIAN miR-96 mimic and scramble controls

为明确前列腺癌（Prostate Cancer, PCa）细胞模型（包含22Rv1的同基因变异株）中的视黄酸受体γ（RARγ）复合物，解析该复合物如何被微小RNA-96（microRNA-96, miR-96）靶向调控、进而调节雄激素受体（Androgen Receptor, AR）顺反组，阐明其在G2/M期细胞中的特异性富集机制，并揭示上述作用如何共同塑造与前列腺癌预后相关的转录程序。实验设计概要：对转染3'端生物素标记的miRIDIAN miR-96模拟物及乱序对照的HPr1AR与LNCaP细胞开展IMPACT-Seq测序。