Gene expression analysis of pancreatic cancer associated fibroblasts

Gene expression analysis of pancreatic cancer associated fibroblasts and control fibroblasts To identify signaling pathways important in tumor-stromal cell interactions, we performed gene expression profiling on pancreatic cancer associated fibroblast cultures and control fibroblast cultures using Affymetrix Exon arrays. We analyzed 3 control fibroblast cultures and 9 pancreatic cancer associated fibroblast cultures using the Affymetrix Human Exon 1.0 ST platform. Gene expression levels were compared using Partek (version 6.3beta).

胰腺癌相关成纤维细胞（pancreatic cancer associated fibroblasts）与对照成纤维细胞（control fibroblasts）的基因表达分析
为鉴定肿瘤-基质细胞互作中的关键信号通路，我们采用Affymetrix外显子阵列（Affymetrix Exon arrays）对两类成纤维细胞的培养物进行了基因表达谱分析。本研究使用Affymetrix人类外显子1.0 ST（Affymetrix Human Exon 1.0 ST）平台，对3株对照成纤维细胞培养物与9株胰腺癌相关成纤维细胞培养物开展检测分析。基因表达水平的比较分析采用Partek（版本6.3beta）完成。