Epigenome analyses reveal histone H4K20me3 and radiation related genes in NSCLC cell

Purpose: Radiotherapy is useful for non-small cell lung cancer (NSCLC) patients who cannot be treated surgically. Modification of histone proteins also occurs during radiotherapy, and affects gene expression. In this study, we assessed the effects of radiotherapy on histone H4K20me3 in NSCLC cells. Methods: NCI-H460 NSCLC cell line were subjected to gamma irradiation. To reveal H4K20me3-related genes, we conducted chromatin immunoprecipitation(ChIP) and ChIP-sequencing. Results: We evaluated the H4K20me3-related genes through ChIP-sequencing. We subjected NCI-H460 cells exposed to 0 or 4 Gy of radiation to ChIP using H4K20me3 antibody (Cell signaling technology,Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb #5737) and sequencing.

研究目的：放射治疗对于无法接受手术治疗的非小细胞肺癌（non-small cell lung cancer, NSCLC）患者具有重要临床价值。放射治疗过程中会发生组蛋白修饰，进而调控基因表达。本研究旨在探讨放射治疗对非小细胞肺癌细胞中组蛋白H4K20me3的影响。
实验方法：将NCI-H460非小细胞肺癌细胞系予以γ射线辐照处理。为筛选与H4K20me3相关的靶基因，本研究采用染色质免疫沉淀（chromatin immunoprecipitation, ChIP）技术及ChIP测序开展相关实验。
实验结果：本研究通过ChIP测序技术筛选得到与H4K20me3相关的靶基因。将经0 Gy或4 Gy剂量射线辐照后的NCI-H460细胞，使用H4K20me3抗体（Cell Signaling Technology公司，三甲基化组蛋白H4（Lys20）(D84D2) 兔单克隆抗体 #5737）进行染色质免疫沉淀后开展测序。