SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in September 2019

Flow cytometry data was collected in September 2019, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs. Since July 2019, sampling has also been conducted at "Gulf" sites: Upper Spencer Gulf (USG) and Gulf St Vincent (GSV), with the original sites identified as "Shelf" sites.

流式细胞术（Flow cytometry）数据于2019年9月在南澳大利亚附近海域采集。

本研究的总体目标是建立南澳大利亚大陆架生态系统的背景知识，以及上升流（upwelling）/盐水流出（saline outflow）事件对微生物群落的影响，最终开发该区域的生物地球化学模型。采样在“南澳大利亚综合海洋系统”（Southern Australian Integrated Marine System, SAIMOS）项目框架下，通过“RV Ngerin”号科考船的航次完成。每次航次中，研究人员对叶绿素荧光最大值（chlorophyll fluorescence maximum, DCM）层的物理、化学和生物特性进行了调查。已采集微微型浮游植物（picophytoplankton）、细菌和病毒的流式细胞术数据。

研究期间共采样了6个主要站点，其中5个位于100米等深线（isobath）上，即RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）和SAM5CB/B5（35.008°S，135.198°E）；另有1个离岸站点B1（36.188°S，136.178°E），位于袋鼠岛西南方向。需要注意的是，结合站点间距离（14-25海里，nautical miles）、陆架中层的平均流速分量（0.01 m/s）和船只平均速度（即9节，knots），表明每个站点采集的是不同的水团。偶尔还会从袋鼠岛的国家参考站（National Reference Station, NRS）（35.832°S，136.447°E）和发生盐水流出的“南澳斯宾塞湾口锚系站”（SA Spencer Gulf Mouth Mooring, SAM8SG）（35.25°S，136.690°E）采集额外样本。自2019年7月起，采样还扩展至“海湾”站点：上斯宾塞湾（Upper Spencer Gulf, USG）和圣文森特湾（Gulf St Vincent, GSV），原站点则被定义为“陆架”站点。