Next Generation Sequencing Facilitates Quantitative Analysis of the effects of ICAM-1 on lung ILC2 Transcriptome. Next Generation Sequencing Facilitates Quantitative Analysis of the effects of ICAM-1 on lung ILC2 Transcriptome

ICAM-1 modulates cytokine expression in ILC2s. While it downregulates the expression of ILC2 key cytokines il5, il13 and il9, it upregulates that of il10. Overall design: mRNA profiles of sorted mouse activated lung ILC2s from C57BL/6 and ICAM-1 mice were generated by deep sequencing, in duplicates, using a NextSeq 500 (Illumina) system.

细胞间黏附分子1（Intercellular Adhesion Molecule 1, ICAM-1）可调控2型固有淋巴细胞（Innate Lymphoid Cells 2, ILC2）的细胞因子表达：其能够下调ILC2核心细胞因子IL-5、IL-13及IL-9的表达，同时上调IL-10的表达。
实验设计：从C57BL/6小鼠与ICAM-1小鼠体内分选获得活化的肺组织ILC2，采用Illumina NextSeq 500测序系统进行双重复深度测序，以获取其mRNA表达谱。