Data underlying the publication: Unjamming transitions in cancer cell spheroids are linked to EMT-dependent cell-matrix interactions

Datasets for the publication "Unjamming transitions in cancer cell spheroids are linked to EMT-dependent cell-matrix interactions", to be submitted.Datasets include the raw tif files of 3D spheroid invasion assays of A549 and MV3 spheroids embedded in collagen (2.4 and 8 mg/mL) and gelMA (30 and 50 mg/mL). This include time-series bright-field imaging for 24 hrs (MV3) and 72 hrs (A549) at one hour interval. The spheroids were treated with exogenous TGF-β and broad spectrum MMP-inhibitor and imaged under same conditions.Datasets include MATLAB/.m codes to analyze the spheroid images for effective spheroid radius, number of disseminated cells, rate of invasion and protrusion length. Additionally, MATLAB codes/.m to plot figures are also included within each respective condition. Raw data for supplementary data is also provided as images, excel files and MATLAB scripts.

本数据集用于待投稿论文《癌细胞球的解阻转变与上皮间质转化（Epithelial-Mesenchymal Transition, EMT）依赖的细胞-基质相互作用相关》。数据集包含A549和MV3细胞球在胶原蛋白（2.4及8 mg/mL）与甲基丙烯酰化明胶（gelMA，30及50 mg/mL）中包埋的3D细胞球侵袭实验原始tif文件，包括MV3细胞球24小时、A549细胞球72小时的时间序列明场成像（时间间隔为1小时）。细胞球经外源性转化生长因子-β（TGF-β）及广谱基质金属蛋白酶（MMP）抑制剂处理，并在相同条件下成像。数据集包含用于分析细胞球图像的MATLAB/.m代码，可计算有效细胞球半径、分散细胞数量、侵袭速率及突起长度；此外，各实验条件组均包含用于绘制图表的MATLAB/.m代码。补充数据的原始数据以图像、Excel文件及MATLAB脚本形式提供。