<i>Actinidia chinensis</i> Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1

Increasing evidence has shown that traditional Chinese medicines and their bioactive components exert an anti-tumor effect, representing a novel treatment strategy. <i>Actinidia chinensis</i> Planch Root extracts (acRoots) have been reported to repress cancer cell proliferation and metastasis. The effect of acRoots on hypopharyngeal carcinoma progression was explored in this study. Firstly, data from MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and colony formation assays showed that incubation with accRoots reduced cell proliferation of hypopharyngeal carcinoma cells. Moreover, acRoots promoted the cell apoptosis of hypopharyngeal carcinoma. Secondly, cell migration and invasion of hypopharyngeal carcinoma cells were suppressed by acRoots. Thirdly, E2F1 (E2F Transcription Factor 1) and lncRNA MNX1-AS1 (MNX1 antisense RNA 1) were up-regulated in hypopharyngeal carcinoma tissues, and reduced in hypopharyngeal carcinoma cells post acRoots incubation. Overexpression of E2F1 attenuated acRoots-induced decrease in MNX1-AS1 in hypopharyngeal carcinoma cells. Lastly, administration with acRoots retarded in vivo hypopharyngeal carcinoma growth through down-regulation of E2F1-mediated MNX1-AS1. In conclusion, acRoots exerted tumor-suppressive role in hypopharyngeal carcinoma through inhibition of E2F1-mediated MNX1-AS1.越来越多的研究证据表明，中药及其生物活性成分可发挥抗肿瘤作用，是一种极具潜力的新型治疗策略。中华猕猴桃（*Actinidia chinensis*）Planch根提取物（acRoots）已有研究报道可抑制癌细胞增殖与转移。本研究旨在探讨acRoots对下咽癌进展的影响。首先，噻唑蓝（MTT，3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）实验与克隆形成实验数据显示，经acRoots孵育可显著降低下咽癌细胞的增殖活性；此外，acRoots可有效促进下咽癌细胞的凋亡。其次，acRoots可显著抑制下咽癌细胞的迁移与侵袭能力。第三，E2F转录因子1（E2F Transcription Factor 1，E2F1）与长链非编码RNA MNX1反义RNA1（lncRNA MNX1-AS1，MNX1 antisense RNA 1）在下咽癌组织中呈高表达状态，而在经acRoots孵育的下咽癌细胞中其表达水平显著下调；过表达E2F1可减弱acRoots诱导的下咽癌细胞中MNX1-AS1水平降低的效应。最后，体内实验结果证实，acRoots可通过下调E2F1介导的MNX1-AS1表达，延缓下咽癌的体内生长。综上，acRoots可通过抑制E2F1介导的MNX1-AS1通路，发挥下咽癌的抑瘤作用。