Computational Analysis of Two-dimensional High-throughput Data from Large-scale RNAi Screens and Single-cell Transcriptomics

This publication provides a singularity definition file to reproduce the computational environment along with the scripts to reproduce every figure or table in the revised manuscript using ZetaSuite Perl module and R package.

First, generate a new folder and then download all the files into the folder.

Then, uncompressed the files DataSets_part1.tar.gz,DataSets_part2.tar.gz,DataSets_part3.tar.gz,DataSets_part4.tar.gz, and scripts.tar.gz. within the folder.

Next, move all the files in DataSets_part1 folder, DataSets_part2 folder,DataSets_part3 folder and DataSets_part4 folder to a new folder called DataSets.

Finally, run the following scripts to generate the figures and tables in our manuscript.

Regeneration of Figure2 and S2: singularity exec ZetaSuite.sif sh Figure2andS2.sh  

Regeneration of Figure3 and S3: singularity exec ZetaSuite.sif sh Figure3andS3.sh  

Regeneration of Figure4 and S4: singularity exec ZetaSuite.sif sh Figure4andS4.sh  

Regeneration of Figure5 and S5: singularity exec ZetaSuite.sif sh Figure5andS5.sh  

Regeneration of Figure6 and S6: singularity exec ZetaSuite.sif sh Figure6andS6.sh  

Regeneration of Figure7 and S7: singularity exec ZetaSuite.sif sh Figure7andS7.sh