Additional file 2 of LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis

Additional file 2: Table S1. gDNA contamination test. Table S2. Oligos of short IVT templates; primers for long template construction and IVT quantification; primer for SCC4 knockout. Table S3. LAST-seq barcoding primers and library indexing primers. Table S4. Primers for A60 spike-ins. Table S5. Quantification of A60 spike-ins.

补充文件2：表S1。基因组DNA（Genomic DNA，gDNA）污染检测。表S2。短体外转录（In Vitro Transcription，IVT）模板寡核苷酸序列；长模板构建及体外转录定量所用引物；SCC4基因敲除引物。表S3。LAST-seq条形码引物及文库索引引物。表S4。A60掺入对照引物。表S5。A60掺入对照的定量分析。