OBLRtrappingdosageClarksvilleForMS.xlsx

Trap captures of obliquebanded leafroller, <i>Choristoneura rosaceana</i> (Harris) to pheromone blends in ratios approximating those reported in pheromone glands and a novel blend based on a volatile headspace collection from live virgin females were evaluated in field experiments in Michigan apple orchards. In an initial field trapping study, pheromone lures composed of either a three- or four-component blend approximation of the blend present in female pheromone glands at doses ranging from 0.1 to 20 mg/lure were compared. The four-component blend was a combination of (<i>Z</i>)-11-tetradecenyl acetate (<i>Z</i>11-14:Ac), (<i>E</i>)-11-tetradecenyl acetate (<i>E</i>11-14:Ac), (<i>Z</i>)-11-tetradecen-1-ol (<i>Z</i>11-14:OH), and (<i>Z</i>)-11-tetradecenal (<i>Z</i>11-14:Al) in a ratio of 96.5:1.8:1.4:0.2, respectively, while the three-component blend lacked <i>Z</i>11-14:Al. Pheromone emissions by groups of virgin females and commercial lures were collected in the laboratory and analyzed by gas chromatography. These data were used to formulate a new pheromone lure that was compared to a commercial lure in a second trapping study. In the first field study, traps baited with 10 mg pheromones or above captured significantly more moths than traps baited with 1 mg or less, regardless of the blend. Surprisingly, groups of virgin females only emitted two detectable pheromone components, <i>Z</i>11-14:Ac and <i>Z</i>11-14:OH in a ratio of 37:63 which was substantially different from the blends detected in pheromone glands in the literature. The newly formulated pheromone lure based on females’ emission was more than twice as attractive as the commercial lure which emitted a 74:5:21 three-component blend of <i>Z</i>11-14:Ac, <i>E</i>11-14:Ac, and <i>Z</i>11-14:OH. Our results suggested that the actual pheromones released by females may not translate directly to the content of pheromone glands.<b></b>

斜纹带卷叶蛾（obliquebanded leafroller，学名*Choristoneura rosaceana* (Harris)）对两种性信息素混合物的田间诱捕效果评估：一种为比例近似雌蛾性信息素腺中已报道组分的混合物，另一种为基于活体处女雌蛾顶空挥发物收集所得的新型混合物，试验在密歇根州苹果园内开展。在初始田间诱捕试验中，研究比较了两种诱芯：一种由三种组分构成，另一种由四种组分构成，二者的组分比例均近似雌蛾性信息素腺内的天然混合物，诱芯剂量范围为0.1至20毫克/诱芯。其中四元混合物为(Z)-11-十四碳烯乙酸酯（(Z)-11-tetradecenyl acetate，Z11-14:Ac）、(E)-11-十四碳烯乙酸酯（(E)-11-tetradecenyl acetate，E11-14:Ac）、(Z)-11-十四碳烯-1-醇（(Z)-11-tetradecen-1-ol，Z11-14:OH）与(Z)-11-十四碳烯醛（(Z)-11-tetradecenal，Z11-14:Al）按96.5:1.8:1.4:0.2的比例混合而成，而三元混合物则不含Z11-14:Al。研究人员在实验室中收集了多组处女雌蛾与商用诱芯的性信息素释放物，并通过气相色谱法进行分析，基于这些数据研制了新型性信息素诱芯，并在第二项诱捕试验中与商用诱芯进行对比。第一项田间试验结果显示，无论使用何种组分混合物，施用10毫克及以上剂量性信息素的诱捕器诱获的蛾类数量均显著高于施用1毫克及以下剂量的诱捕器。令人意外的是，处女雌蛾仅能释放两种可检测到的性信息素组分——Z11-14:Ac与Z11-14:OH，比例为37:63，这与文献中报道的雌蛾性信息素腺内的混合物比例差异显著。基于雌蛾释放组分研制的新型诱芯，其诱集活性是商用诱芯的两倍以上；该商用诱芯释放的三元混合物为Z11-14:Ac、E11-14:Ac与Z11-14:OH，比例为74:5:21。本研究结果表明，雌蛾实际释放的性信息素组分比例，未必与雌蛾性信息素腺内的组分含量直接对应。