Supporting data for "The role and mechanisms of interleukin-9 in facilitating anticancer effect of immune checkpoint blockade in lung cancer animal model".

This dataset includes the data used in thesis entitled "The role and mechanisms of interleukin-9 in facilitating anticancer effect of immune checkpoint blockade in lung cancer animal model". <br> Laboratory research data includes: 1) Western blot: Images will be scanned and saved as .jpg or .tif files. Measurements and quantification of images can be recorded into excel spreadsheets. 2) MTT: MTT datasets are exported as .xlsx excel files, showing the OD values at 570nm and the test date. 3) Tumor volume will be recorded every other day as three diameters in a .xlsl file, documenting the treatment time and groups. 4) Flow cytometry: Raw flow cytometry files saved from Cotyflex will be in the format of .fcs. Fowjo is used to analyze the .fcs files. Final datasets will be saved as a .wsp file and images will be around 500MB. 5) RT-qPCR: Real-time exponential graphs and data generated by the StepOne program will be exported as .csv excel files and .eds raw data files. Files generated throughout the entire course will be approximately 1-1.5 GB. 6) Statistical analysis software includes GraphPad Prism and ImageJ, files will be saved in their respective intrinsic/proprietary formats.

本数据集包含题为《白细胞介素-9（interleukin-9）在促进肺癌动物模型中免疫检查点阻断抗癌效应中的作用及机制》的论文所用数据。<br>实验室研究数据包括：1）蛋白质印迹法（Western blot）：图像将被扫描并保存为.jpg或.tif文件，图像的测量及定量结果可记录至Excel表格中；2）MTT法（MTT）：MTT数据集以.xlsx格式导出，显示570nm处的OD值（optical density）及测试日期；3）肿瘤体积每隔一天记录三个直径至.xlsl文件，记录治疗时间及组别；4）流式细胞术（flow cytometry）：从Cotyflex保存的原始流式细胞术文件为.fcs格式，使用Fowjo软件分析.fcs文件，最终数据集保存为.wsp文件，图像大小约500MB；5）实时定量聚合酶链式反应（RT-qPCR）：StepOne程序生成的实时指数图及数据以.csv格式和.eds原始数据文件导出，整个过程生成的文件大小约为1-1.5 GB；6）统计分析软件包括GraphPad Prism和ImageJ，文件保存为各自的固有/专有格式。