Data from: Specific membrane capacitance, cytoplasm conductivity and instantaneous Young’s modulus of single tumour cells

As label-free biomarkers, biophysical properties of cells are widely used for cell type classification. However, intrinsic biophysical markers, e.g. specific membrane capacitance (Cspecific membrane), cytoplasm conductivity (σconductivity) and instantaneous Young’s modulus (Einstantaneous) measured for hundreds of single cells were not yet reported. In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25°C, and the entry processes and impedance profiles were recorded and translated to Cspecific membrane, σconductivity and Einstantaneous. Cspecific membrane, σconductivity and Einstantaneous of five cell types were quantified as 2.10±0.38 μF/cm2, 0.91±0.15 S/m and 5.52±0.95 kPa for H460 cells (ncell=437); 2.52±0.54 μF/cm2, 0.83±0.12 S/m and 5.54±1.04 kPa for H446 cells (ncell=410); 2.45±0.57 μF/cm2, 0.99±0.18 S/m and 5.16±1.68 kPa for A549 cells (ncell=442); 1.86±0.31 μF/cm2, 1.07±0.18 S/m and 3.86±0.81 kPa for 95D cells (ncell=415); 2.03±0.35 μF/cm2, 0.99±0.16 S/m and 3.49±0.70 kPa for 95C cells (ncell=290). The database of Cspecific membrane, σconductivity and Einstantaneous may serve as a reference for future studies of cellular biophysical properties.

细胞生物物理特性作为无标记生物标志物，被广泛应用于细胞类型分类任务。然而，此前尚未有针对数百个单细胞测得的固有生物物理标志物——比膜电容（specific membrane capacitance，Cspecific membrane）、胞质电导率（cytoplasm conductivity，σconductivity）以及瞬时杨氏模量（instantaneous Young’s modulus，Einstantaneous）——的相关报道。本研究中，将经胰蛋白酶消化处理的贴壁细胞制备为悬浮单细胞悬液，在25℃条件下通过微流体收缩通道进行抽吸，并记录细胞进入通道的动态过程与阻抗图谱，进而将采集数据转换得到上述三类生物物理参数的数值。本研究对5种细胞的上述三类生物物理参数进行了定量分析，具体结果如下：H460细胞（n=437）的比膜电容、胞质电导率与瞬时杨氏模量分别为2.10±0.38 μF/cm²、0.91±0.15 S/m与5.52±0.95 kPa；H446细胞（n=410）分别为2.52±0.54 μF/cm²、0.83±0.12 S/m与5.54±1.04 kPa；A549细胞（n=442）分别为2.45±0.57 μF/cm²、0.99±0.18 S/m与5.16±1.68 kPa；95D细胞（n=415）分别为1.86±0.31 μF/cm²、1.07±0.18 S/m与3.86±0.81 kPa；95C细胞（n=290）分别为2.03±0.35 μF/cm²、0.99±0.16 S/m与3.49±0.70 kPa。本研究所构建的该三类生物物理参数数据库，可为后续细胞生物物理特性相关研究提供参考依据。