Microarray analysis of Dorsal root ganglion (DRG) sensory neurons from the liver kinase B1 (LKB1) knockout

The goal of this study is to uncover the changes in the transcriptome of sensory neurons of the liver kinase B1 (LKB1) knockout Methods: mRNA profiles of Dorsal root ganglion (DRG) sensory neurons directly isolated from E13.5 embryos were generated. 5 independent replicates of RNA purification of each genotype were prepared, each replica is of DRGs from 2 embryos本研究旨在揭示肝激酶B1（liver kinase B1，LKB1）敲除的感觉神经元的转录组变化。方法：从13.5天胚胎中直接分离背根神经节（Dorsal root ganglion, DRG）感觉神经元并制备其mRNA表达谱。每种基因型设置5次独立的RNA纯化生物学重复，每个重复使用来自2枚胚胎的背根神经节组织。