CIL:38666, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and cation-dependent mannose-6-phosphate receptor (an integral membrane protein that localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane; smaller gold particles) staining. Note that trans‚ÄêGolgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.

本数据集采用免疫电子显微镜（immunoelectron microscopy）技术，对泌乳大鼠的乳腺组织进行成像分析，旨在探究成熟脂滴与反式高尔基体网络（trans-Golgi network）各组分之间潜在的结构相互作用；反式高尔基体网络的相关组分通过TGN38（一种跨膜反式高尔基体蛋白，对应较大金颗粒）与阳离子依赖型甘露糖-6-磷酸受体（一种定位于反式高尔基体网状结构、内体及质膜的整合膜蛋白，对应较小金颗粒）的免疫染色进行识别。
注：泌乳乳腺细胞内的反式高尔基体潴泡（trans-Golgi cisternae）含有大量酪蛋白与其他乳源蛋白，且因分泌液的渗透压作用而发生显著扩张。