Identification of DAXX As A Restriction Factor Of SARS-CoV-2 Through A CRISPR/Cas9 Screen

While the interferon response restricts SARS-CoV-2 replication in cell culture, only a handful of Interferon Stimulated Genes with antiviral activity against SARS-CoV-2 have been identified. Here, we describe a functional CRISPR/Cas9 screen aiming at identifying SARS-CoV-2 restriction factors. We performed a CRISPR/Cas9 screen specifically designed to identify restriction factors for SARS-CoV-2, assessing the ability of 1905 ISGs to modulate SARS-CoV-2 replication in human epithelial lung cells.