Hyphthalamic mRNA expression of leptin receptor-expressing cell-specific Slug knockout mice

Leptin resistance is a driving force for obesity and metabolic syndrome. Slug (Snai2 or Snail2) is an epignetic factor that may play a critical role in the development of leptin resistance in obesity. We conducted a microarray to detect molecular targets of Slug in the hypothalamus. Hypothalamic RNAs were extracted from leptin receptor-expressing cell-specific Slug knockout (ΔLepR) or Slugfl/fl male mice at 8 weeks of age and subjected to Affymetrix microarray analysis.