Data from: Development of HPLC-ELSD method for determination of phytochelatins and glutathione in Perilla frutescens under cadmium stress condition

A rapid, accurate and simple method was developed for the simultaneous determination of glutathione (GSH) and phytochelatins (PCs) by high performance liquid chromatography (HPLC) with evaporative light-scattering detector(ELSD). GSH, Phytochelatin 2 (PC2), phytochelatin 3 (PC3), phytochelatin 4 (PC4), phytochelatin 5 (PC5) and phytochelatin 6 (PC6) can be separated with baseline separation within 9 minutes using a Venusil AA column (250 mm × 4.6 mm i.d., 5 μm particle sizes). The acetonitrile (A) and water containing 0.1% trifluoroacetic acid (0.1% TFA, B) were employed as the mobile phase for the gradient elution. The drifttube temperature and flow rateofcarriergas (N2) were 50℃ and 1.5 L·min-1, respectively. Under the optimum conditions, good linear regression equations of six analytes were obtained with the detection limits ranging from 0.2 to 0.5 µg·mL-1. The proposed method has been applied successfully for the quantification of GSH and PCs in Perilla frutescens (a cadmium hyperaccumulator) under cadmium stress. The recoveries were between 82.9% and 115.3%.

本研究建立了一种快速、准确且简便的分析方法，可采用高效液相色谱法（HPLC）结合蒸发光散射检测器（ELSD）同时测定谷胱甘肽（GSH）与植物螯合肽（PCs）。使用Venusil AA色谱柱（250 mm × 4.6 mm 内径，粒径5 μm），可在9分钟内实现谷胱甘肽、植物螯合肽2（PC2）、植物螯合肽3（PC3）、植物螯合肽4（PC4）、植物螯合肽5（PC5）及植物螯合肽6（PC6）的基线分离。以乙腈（A相）和含0.1%三氟乙酸（0.1% TFA，B相）的水溶液作为流动相进行梯度洗脱。载气（N₂）的漂移管温度与流速分别为50℃与1.5 L·min⁻¹。在最优实验条件下，六种被测物均获得了良好的线性回归方程，检出限范围为0.2 ~ 0.5 µg·mL⁻¹。本方法已成功应用于镉胁迫下镉超富集植物紫苏（Perilla frutescens）中谷胱甘肽与植物螯合肽的定量分析，加标回收率介于82.9% ~ 115.3%之间。