Changes in gene expression within the skin caused by antigen-specific memory CD4+ T cells during Vaccinia virus infection.

To investigate the mechanisms used by memory CD4+ T cells to protect the skin from poxvirus infection, we infected LCMV immune mice with VacV on the right ear skin and VacV expressing the dominant MHC-II restricted epitope from LCMV (VacV-Ii-GP61) on the left ear skin. On day 3 after the co-infection, RNA from whole skin was isolated and gene expression was analyzed using RNA sequencing. Comparative gene expression profiling analysis of RNA-seq data from VacV-infected skin compared to VacV-Ii-GP61 infected skin.